Table 1.
Regression analysis for the determination of nine analytes in intra-day and inter-day analysis#.
| Compound (Peak No.) | Retention time (min) | Calibration range (μg/mL) | Regression equation | Coefficient of correlation (R2) |
|---|---|---|---|---|
| Resveratroloside (Res, 1) | 4.83 | 0.75−12.00 | Y = 13280.0X + 3543.1 | 0.9931 |
| Sennoside A (Se-A, 2) | 5.58 | 0.40−6.40 | Y = 42404.0X + 3029.2 | 0.9971 |
| Baicalin (Ba, 3) | 10.91 | 5.00−80.00 | Y = 37233.0X + 52341 | 0.9983 |
| Coptisine (Co, 4) | 18.42 | 0.10−2.40 | Y = 55585.0X + 5375.3 | 0.9903 |
| Palmatine (Pa, 5) | 19.58 | 0.25−4.00 | Y = 13039.0X + 2777.9 | 0.9916 |
| Berberine (Be, 6) | 21.13 | 0.75−12.00 | Y = 73590.0X − 1532.2 | 0.9986 |
| Rhein (Rh, 7) | 23.81 | 0.10−1.60 | Y = 99814.0X + 8013.8 | 0.9939 |
| Aloe-emodin (Ale, 8) | 24.37 | 0.075−1.200 | Y = 75638.0X + 1326.2 | 0.9975 |
| Wogonin (Wo, 9) | 25.12 | 0.025−0.600 | Y = 90308.0X + 2655.5 | 0.9954 |
#
The regression equations for inter-day analysis were calculated from the assay values of the prepared standards on three different days (n = 3).