. 2016 Oct 20;7:374. doi: 10.3389/fphar.2016.00374

Table 3.

Precision and accuracy for the determination of nine analytes in intra-day and inter-day^#.

Compound (Peak No.)	Intra-day analysis (n = 3)			Inter-day analysis (n = 3)			Accuracy (%)
	Concentration known^a (μg/mL)	Concentration found (μg/mL)	R.S.D (%)	Concentration known^a (μg/mL)	Concentration found (μg/mL)	R.S.D (%)
RESVERATROLOSIDE (RES, 1)
	3	3.11 ± 0.14	4.37	3.56	2.98 ± 0.01	0.34	−0.67
	6	6.89 ± 0.62	9.01	14.78	6.53 ± 0.04	0.62	8.78
	12	12.09 ± 0.25	2.10	0.72	12.17 ± 0.18	1.50	1.44
SENNOSIDE A (SE-A, 2)
	1.6	1.49 ± 0.09	6.08	−6.67	1.70 ± 0.03	1.48	6.46
	3.2	3.23 ± 0.02	0.47	0.83	3.15 ± 0.01	0.32	−1.56
	6.4	6.38 ± 0.01	0.16	−0.31	6.46 ± 0.14	2.10	0.89
BAICALIN (BA, 3)
	20	19.87 ± 0.12	0.58	−0.63	21.58 ± 0.14	0.66	7.29
	40	41.17 ± 0.31	0.76	2.92	40.30 ± 0.67	1.66	0.76
	80	80.71 ± 0.75	0.93	0.89	82.50 ± 0.02	0.03	2.57
COPTISINE (CO, 4)
	0.6	0.52 ± 0.02	2.96	3.56	0.57 ± 0.01	1.75	5.00
	1.2	1.31 ± 0.04	3.19	14.78	1.25 ± 0.03	2.44	4.44
	2.4	2.36 ± 0.01	0.42	0.72	2.51 ± 0.12	4.71	4.72
PALMATINE (PA, 5)
	1	0.90 ± 0.07	7.78	−6.67	1.07 ± 0.02	1.42	7.33
	2	2.09 ± 0.02	0.73	0.83	2.09 ± 0.14	6.78	4.33
	4	4.39 ± 0.30	6.83	−0.31	4.04 ± 0.06	1.51	1.00
BERBERINE (BE, 6)
	3	3.25 ± 0.16	4.77	−0.63	3.06 ± 0.08	2.47	2.11
	6	6.09 ± 0.16	2.58	2.92	6.20 ± 0.07	3.28	3.28
	12	12.24 ± 0.02	0.17	0.89	12.08 ± 0.12	0.69	0.69
RHEIN (RH, 7)
	0.4	0.41 ± 0.02	1.67	3.56	0.37 ± 0.01	1.55	−6.67
	0.8	0.81 ± 0.02	0.83	14.78	0.81 ± 0.01	1.23	1.25
	1.6	1.63 ± 0.01	1.87	0.72	1.60 ± 0.02	0.95	0.21
ALOE-EMODIN (ALE, 8)
	0.3	0.29 ± 0.02	−4.44	−6.67	0.30 ± 0.01	1.95	−1.11
	0.6	0.64 ± 0.02	7.22	0.83	0.62 ± 0.01	1.61	3.33
	1.2	1.22 ± 0.01	1.67	−0.31	1.22 ± 0.02	1.64	1.67
WOGONIN (WO, 9)
	0.15	0.17 ± 0.01	11.11	−0.63	0.13 ± 0.01	7.69	−13.33
	0.3	0.32 ± 0.01	7.78	2.92	0.34 ± 0.02	4.45	14.44
	0.6	0.58 ± 0.01	−2.78	0.89	0.61 ± 0.01	1.64	1.67

Intra-day assay variance was validated with three known different concentrations of analytes at five intervals at a single day and inter-day assay variance was validated with three known different concentrations of analytes during three successive days.