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Optimization of a luminescence-based HTS assay against eEF2K. (A) Reaction time. (B) Enzyme concentration and room temperature storage time. (C) Z′ factor of the assay was determined at the optimized assay conditions. The background (closed circles) represents luminescence in 2% DMSO (negative control) and the signal (open circles) indicates that in 25 µmol/L of A484954 (positive control). Values given in A and B are means±SEM. RLU, relative luminescence unit.
