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. 2016 May 21;6(6):557–563. doi: 10.1016/j.apsb.2016.04.002

Figure 4.

Fig. 4

Western blot analysis of hit compounds in cancer cells. (A) Effect of WNN0048-A002 on eEF2K in MDA-MB-453 cells. The breast cancer cell line was treated with different concentrations of the compound for 6 h. Cell lysates were analyzed by western blotting using antibodies specific for phospho-eEF2 (Th-56). β-Actin was used as a loading control. P-eEF2, eEF2 phosphorylation. (B) Histogram of the western blotting analysis data shown as mean ±SEM from three independent experiments in duplicate.