Figure 4.

Functional tests of mouse embryonic stem cell (mESC)‐derived hepatocyte‐like cells (iHeps) induced by GF‐PEI‐MSN complexes, PEI‐MSNs, growth factors alone, and without treatment. A) The functions of glycogen synthesis and storage were measured by periodic acid‐Schiff (PAS) assays on the mESC‐derived cells in the four groups. Glycogen storage is indicated by pink or dark red‐purple cytoplasms. B) The cellular uptake function of indocyanine green (ICG), and C) low‐density lipoprotein (LDL) in the different treatment groups was analyzed using a fluorescence microscope at the end of the differentiation process. Scale bar: 50 μm.