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. 2016 Apr 7;58(8):749–758. doi: 10.1111/jipb.12467

Figure 2.

Figure 2

The effect of constitutive expression of ZmAGO1a in ago1‐27 of Arabidopsis (A) ZmAGO1a gene structure. Empty rectangles, untranslated region (5′ or 3′ UTR); Black rectangles, exons; Fold lines, introns; 8451bp, total genomic DNA length; AGPv3:6:43253105:43261555:1, maize genome ver3 physical region annotation. (B) The p35S::ZmAGO1a construct in which the ZmAGO1a cDNA was expressed under the control of CaMV35S promoter (35S) and NOS terminator (Tnos). Hollow arrows with the text “35S” inside indicated CaMV35S promoter. Bar, glufosinate ammonium resistance selection marker; 35S‐PloyA, CaMV35S polyA signal sequence; LB and RB, T‐DNA left and right borders. Vertical arrows indicated the restriction sites. (C) Phenotypic comparison of seedlings among the wild type, ago1‐27 and ago1‐27 carrying p35S::ZmAGO1a construct. (D) Phenotypic comparison at the flowering stage among the wild type, ago1‐27 and ago1‐27 carrying p35S::ZmAGO1a construct (note: the ago mutant flowered later). (E) Phenotypic comparison at the mature state among the wild type, ago1‐27 and ago1‐27 carrying p35S::ZmAGO1a construct (note: the ago mutant matured later.) C–E: wt, wild type Arabidopsis thaliana (L.) Colombia (Col‐0); ago1‐27, Arabidopsis thaliana (L.) ago1‐27 mutant in the Col‐0 background; line 16, 19, and 21: three independent homozygous ago1‐27 T3 lines transformed with 35S::ZmAGO1a.