Table 2. Revertants/plate, standard deviation and mutagenicity index (in brackets) for the strains TA98, TA100, TA102 and TA97a of S. typhimurium after treatment with various doses of phytochemical Betulin with (+S9) metabolic activation .
|
Treatments
(mg/plate) |
Number of revertants (M ± SD)/plate and MI | ||||
| TA 98 | TA 100 | TA 102 | TA 97a | ||
| + S9 | + S9 | + S9 | + S9 | ||
| Betulin | 0.0 a | 24 ± 9 | 107 ± 20 | 205 ± 11 | 84 ± 16 |
| 0.63 | 27 ± 4 (1.1) | 117 ± 6 (1.1) | 219 ± 19 (1.1) | 102 ± 24 (1.2) | |
| 1.25 | 27 ± 7 (1.1) | 97 ± 5 (0.9) | 199 ± 21 (1.0) | 84 ± 6 (1.0) | |
| 2.50 | 29 ± 3 (1.2) | 105 ± 15 (1.0) | 223 ± 38 (1.1) | 80 ± 15 (0.9) | |
| 3.75 | 27 ± 1 (1.1) | 95 ± 17 (0.9) | 240 ± 27 (1.2) | 71 ± 16 (0.8) | |
| 5.00 | 37 ± 5 (1.5) | 94 ± 17 (0.9) | 183 ± 21 (0.9) | 74 ± 13 (0.9) | |
| Control + | 1551 ± 81e | 2627 ± 297e | 1043 ± 39f | 1252 ± 30e | |
M ± SD = mean and standard deviation; MI = mutagenicity index; aNegative control: dimethylsulfoxide (DMSO - 50 μL/ plate); Control+ = Positive control - e2-anthramine (1.25 μg/ plate – TA 97a, TA98, TA100); f2-aminofluorene (10.0 μg/ plate – TA102), in the presence of S9.