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. 2016 Oct 20;6:35668. doi: 10.1038/srep35668

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Copyright © 2016, The Author(s)

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(A) cDNAs from the winged S. avenae were used for template. Lane 1: 100 bp ladder marker; Lane 2: miR-315; Lane 4: miR-1; Lane 6: miR-9a; Lane 8: PC-5p-113190_15; Lane 10: PC-3p-2743_844; Lane 12: miR-7; Lane 14: miR-8; Lane 16: miR-277; Lane 18: Let-7. The other uneven lanes were negative controls for each target miRNA. The relative miRNA expression, including miR-277 (B), miR-1 (C), miR-7 (D), Let-7 (E), miR-9a (F), miR-315 (G), miR-8 (H), PC-5p-113190_15 (I), and PC-3p-2743_844 (J) at two wing morph was normalized to the wingless adult. W: winged adult; WL: wingless adult. *P < 0.05; **P < 0.01; ***P < 0.001. Full-length gel is presented in Supplementary Figure S4.