Figure 4. Caspase-9, Caspase-3 activation and PARP cleavage upon AKT inhibitors incubation.

Cleavage and activation of initiator Caspase-9, effector Caspase-3 and PARP proteolysis (Caspase-3 substrate) were analyzed by Western blot in H9 and FN2.1 cells at 2, 4, 8, 16 and 24 hours post specific AKT inhibitors treatment [AKTi IV (IV, 1 μM), AKTi VIII (VIII, 10 μM) and GSKi (GSK, 1 μM)]. Actin was used as loading control. Representative blots of three independent experiments are shown.