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. 2016 Oct 20;6:35475. doi: 10.1038/srep35475

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Copyright © 2016, The Author(s)

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(a) Isoproterenol significantly enhanced Kv7.1 current whereas puerarin (100 μM) antagonized such activation effect. (b) Forskolin significantly enhanced Kv7.1 current whereas puerarin (100 μM) antagonized such activation effect. (c) 8-Br-cAMP significantly enhanced Kv7.1 current whereas puerarin (100 μM) antagonized such activation effect. (d) 1 mM puerarin had no effect on intracellular cAMP concentration and no effect upon the increase of cAMP induced by isoproterenol, forskolin in rat cardiac myocytes. (e) forskolin significantly enhanced IKs current whereas puerarin (100 μM) antagonized such activation effect in rat cardiac myocytes. (f ) Statistical analysis of Fig. 5e.