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. 2016 Oct 20;6:35546. doi: 10.1038/srep35546

Figure 7. Expression of key genes involved in ecdysone, JH and nutrition signaling in the ovary and fat body.

Figure 7

To study the effect of feeding and mating on the expression of these genes, four different treatments F+M+, adults fed and mated; F+M−, adults fed but not mated; F−M+, adults not fed, but mated; and F−M−, adults neither fed nor mated were included. The insects were dissected at day 0, day 2 and day 3 after feeding, total RNA was isolated from the fat body and ovaries. X-axis shows treatment groups and time, day 0 (black), day 2 (pattern), day 3 (gray) and the y-axis shows the relative mRNA levels. Relative mRNA expression of vitellogenin1 (Vg1), vitellogenin2 (Vg2), vitellogenin3 (Vg3), methoprene-tolerant protein (Met), krüppel homologue 1 (Kr-h1), ecdysone receptor (EcR), hormone receptor 3 (HR3), insulin peptide 1 (ILP1), insulin peptide 2 (ILP2), were determined after normalization with ribosomal protein 8 (rpl8). Data shown are mean + SD (n = 3). (Alphabet represents significance at 95% CI).