Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 20;6:35203. doi: 10.1038/srep35203

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) endoTrpV1 (top, green) and tdTom (bottom, red) sdFISH in TrpV1-CretdTom P3 mice. 73% of endoTrpV1 neurons are tdTom positive, 36% of tdTom cells contain endoTrpV1 at P3 (right). (b) Dat (green) and tdTom sdFISH. tdTom is restricted to the medial midbrain and labels a medial area in PBP low in dopaminergic neurons (szPBP). (c) Overview of endoTrpV1-only (green dots), tdTom-only (red dots) and co-expressing cells (white dots) from rostral to caudal midbrain. (d) Electrophysiological characterization of TrpV1-cre^tdTom cells in P10-18 TrpV1-cre^tdTom mice. Current-clamp recordings at different holding currents (top left); firing frequency during current injection (n = 10) (top right); Voltage-clamp recordings of I_h in I_h- and I_h+ cells (bottom left); Mean I_h at −132 mV holding potential in I_h- (n = 4) and I_h+ (n = 11) cells (bottom right). (e) Th (green) and tdTom sdFISH (red dots) in adult mice. tdTom labels a population of neurons rostral of the IF and caudal of the PH labeled rmVTA (top). (f) sdFISH of tdTom (red) with Dat (first column), Th (second column), Viaat (third column) and Vglut2 (fourth column) (green) in the IF (first row), PBP (second row) and szPBP (third row). (g,h) Manual cell-counting results of Dat-, Th-, Viaat and Vglut2-coexpression with tdTom in the VTA (G, subnuclei values in H) and SuM (H) in (n = 3) adult mice. All sdFISH images were cropped from whole slides acquired with a digital high resolution slide scanner. Abbreviations: CLi, caudal linear nucleus; DR, dorsal raphe nucleus; sdFISH, mRNA-selective double-fluorescent in situ hybridization; tdTom, tdTomato; endoTrpV1, endogenous TrpV1; IF, interfascicular nucleus; I_h, hyperpolarization-activated current; PAG, periaqueductal grey; PH, posterior hypothalamus; PBP, parabrachial nucleus + ventral tegmental area rostral part; PN/PIF, paranigral nucleus + parainterfascicular nucleus; RLi, rostral linear nucleus; rmVTA, rostromedial ventral tegmental area; SNc, substantia nigra pars compacta; SuM, supramammillary nucleus; VTA, ventral tegmental area.