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. 2016 Oct 20;6:35764. doi: 10.1038/srep35764

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Copyright © 2016, The Author(s)

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For each titration, β-cyclodextrin concentration in sample cell was taken as 50 μM and nicotinic acid concentration in syringe was 500 μM. The top panel represents the raw heats of binding obtained upon titration of nicotinic acid to β-cyclodextrin. The lower panel is the binding isotherm fitted to the raw data using one site model.