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. 2016 Oct 18;26(10):1059–1071. doi: 10.1093/glycob/cww053

Fig. 4.

Fig. 4.

Effect of compound 1 (1 mM) on PSGL-1 cell surface levels. Monocytes from whole blood were incubated for 0, 24, 48 and 72 hours with or without IL-1β (10 ng/mL), and with or without compound 1. Samples were analyzed using flow cytometry. Graphs show (A) the percentage of mononuclear cells displaying cell surface PSGL-1, (B) the mean fluorescence intensity (MFI) of PSGL-1 cell surface levels. Data are expressed as mean ± SEM (n = 4–6). *P < 0.01 and **P < 0.05 between IL-1β-stimulated groups.