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Neuraminidase treatment. Monocytes from whole blood were incubated for 72 hours with or without IL-1β (10 ng/mL). Samples were treated with or without neuraminidase (0.025–0.1 U/mL) and analyzed using flow cytometry. Graphs show (A) the MFI of sLeX cell surface levels, and (B) the MFI of PSGL-1 cell surface levels. Data are expressed as mean ± SEM (n = 4–6).
