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. Author manuscript; available in PMC: 2017 Oct 18.
Published in final edited form as: Chem Commun (Camb). 2016 Oct 18;52(85):12606–12609. doi: 10.1039/c6cc05959g

Figure 2. The genetic incorporation of fluorophenylalanines.

Figure 2

(A) Structures of five fluorophenylalanines. (B) The expression of sfGFP with fluorophenylalanines incorporated at its S2 position. To express full-length sfGFP, E. coli BL21 cells were transformed with two plasmids coding PylRS-AS, tRNAPyl, and sfGFP2TAG and the transformed cells were grown in the GMML medium supplemented with or without a fluorophenylalanine at 3 mM. (C) Deconvulated ESI-MS spectra of purified full-length sfGFP proteins. Theoretical molecular weights are 27819, 27801, 27783, 27765, and 27765 Da for sfGFP-F5F, sfGFP-F4F, sfGFP-F3F, sfGFP-F2F, and sfGFP-F2F’, respectively.