Table 1.
VNTR | Length TR | Forward primer | Length 5′ | Reverse primer | Length 3′ |
---|---|---|---|---|---|
VNTR A* | 19 | GAGGGATATACCCCACCTCTTT | 5 | GGGGAAGTATGATCCCGAAG | 17 |
VNTR B* | 21 | CCGGAATAATCCGCTTATGA | 22 | ATCACCAGAGTTGGCGATTC | 3 |
VNTR C* | 24 | TGGTTTAGGAACCGGTGTTG | 47 | CACATTAAAGCCTGTGCAGGTA | 38 |
VNTR D* | 24 | ATCATGGCGGTTGGGATG | 2 | CACAGGCTCGACAACCACTA | 13 |
VNTR E* | 68 | TGTTCAATTTTGATTGTTTTGTTCA | 73 | TATATGGCGGTCGGCTTAAT | 2 |
VNTR F | 48 | TACCCCAATCTGCCTTGTTG | 70 | CATGCTCCTGCGTGGTATAA | 41 |
VNTR G | 18 | GTCATTACGGCCCAGGTG | 20 | TGAGGCTGCAAAGACAGATG | 22 |
Five VNTR loci (depicted with *) were used to combine in the multiplex PCR. Of each VNTR locus the length, forward primer and reverse primer are given. The distance (in base pairs) between the primer annealing site and the tandem repeat was mentioned as length 5′ and length 3′.