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. 2016 Jul 1;9(6):772–781. doi: 10.1111/1751-7915.12375

Table 1.

VNTR primer sequences

VNTR Length TR Forward primer Length 5′ Reverse primer Length 3′
VNTR A* 19 GAGGGATATACCCCACCTCTTT 5 GGGGAAGTATGATCCCGAAG 17
VNTR B* 21 CCGGAATAATCCGCTTATGA 22 ATCACCAGAGTTGGCGATTC 3
VNTR C* 24 TGGTTTAGGAACCGGTGTTG 47 CACATTAAAGCCTGTGCAGGTA 38
VNTR D* 24 ATCATGGCGGTTGGGATG 2 CACAGGCTCGACAACCACTA 13
VNTR E* 68 TGTTCAATTTTGATTGTTTTGTTCA 73 TATATGGCGGTCGGCTTAAT 2
VNTR F 48 TACCCCAATCTGCCTTGTTG 70 CATGCTCCTGCGTGGTATAA 41
VNTR G 18 GTCATTACGGCCCAGGTG 20 TGAGGCTGCAAAGACAGATG 22

Five VNTR loci (depicted with *) were used to combine in the multiplex PCR. Of each VNTR locus the length, forward primer and reverse primer are given. The distance (in base pairs) between the primer annealing site and the tandem repeat was mentioned as length 5′ and length 3′.