Figure 2.

Growth curve of CR0010 organoid spheroids within 25 days. We set four cell densities. Under these growth conditions, cells all formed organoids and grew over time. With densities of 500 cells/well and 1000 cells/well in ultra-low attachment 96-well plates, the cells barely grew and formed spheroids with effective diameter. The other two densities, 2000 cells/well and 4000 cells/well, displayed vigorous growth rate. The organoids with 2000 cells/well showed double the growth of 4000 cells/well (8 vs. 4).