Fig. 1.

Identification of the BCR-ABL and ABL-BCR fusions in chemically degraded RNA from a KU812 cell line using the TruSeq RNA-seq protocol. a Supporting reads per million reads for BCR-ABL and ABL-BCR at different RIN values (10, 7, 5, 3). b Diagram of ABL and BCR genes and the BCR-ABL and ABL-BCR fusion products showing the different distances of each fusion product from the 3′ end. c Coverage level per million reads as a function of the distance from the 3′ end for the ABL-BCR fusion at different levels of degradation. A loess trend line is depicted for each sample. d Coverage level per million reads as a function of the distance from the 3′ end for the BCR-ABL fusion at different levels of degradation. A loess trend line is depicted for each sample