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. 2016 Oct 19;48:79. doi: 10.1186/s12711-016-0257-2

Fig. 4.

Fig. 4

Verification of the duplication by PCR in animals with different genotypes at rs110675140. The upper band (370 bp) is an internal control that is amplified in all animals, while the lower band (221 bp) is the fragment that is amplified in animals carrying the duplication. GA, GG, and AA are the genotypes of the animals at rs110675140. Lane 10 is a negative control, L is the 500 bp reference ladder