Fig. 4.

Dehydration interrupted renal morphogenesis by regulating Shh signaling in infant mice. a Top disease and functional categories of gene sets among differentially expressed genes in dehydrated mice during infancy. b Gene set enrichment analysis (GSEA) was used to evaluate the most significant functional category of differentially expressed genes by dehydration in infant mice and generate enrichment plot of cell cycle-related genes. c The heatmap shows downstream target genes of the SHH signaling pathway and indicates that cell cycle and cell differentiation regulators were distinctively regulated by dehydration. d Western blot assay was conducted for SHH signaling members. e The transcript level of its target molecules was validated with qPCR analysis. Data are expressed as mean ± SEM. Student’s t test; *p < 0.05 versus control group