Figure 5.
Tau45-230 was not detected in the spinal cord of SOD1 transgenic mice. (AC) Western blot analysis of cervical (SCC) and lumbar (SCL) spinal cord samples from wild-type (WT) and SOD1 transgenic (SOD1) mice obtained 10 and 17 wks after birth reacted with tau (clone tau5) (A and B) and tau45-230 (C) antibodies. No immunoreactive bands of 17 kDa apparent molecular weight were detected in any of the samples analyzed. A tau45-230-immunoreactive band was only detected in whole-cell extracts prepared from hippocampal cultures treated with aggregated β amyloid (Aβ) used as a positive control (C). Class III β tubulin (TUJ1) was used as loading control.