Figure 2.

Effect of AOAA and YD0171 on the H₂S-synthesizing activity of human recombinant full-length CBS enzyme. AOAA concentration dependently inhibited H₂S production (detected by the AzMC method) with an IC₅₀ of ~2 μmol/L, while YD0171 was a substantially weaker direct inhibitor of recombinant CBS activity (IC₅₀: ~300 μmol/L). Data shown as mean ± SEM; n = 3.