Figure 8.

Reciprocal gene regulatory patterns in DH and DHTO. (A) Hierarchical clustering of genes increased in DHTO and decreased in DH yielded clusters similar to clustering using all genes except that in this analysis one TO sample was most similar to DHTO and one TO sample was most similar to DH. The expression of genes in the control group was generally between that of DHTO and DH. (B) Pathway analysis of genes downregulated in DH and upregulated in DHTO revealed that the top ten upregulated pathways and processes were all downstream of epidermal growth factor receptor or ephrin signaling. The top fifteen pathways downregulated in DH and upregulated in DHTO all involved protein synthesis or trafficking. (C) The ten most significantly altered processes downregulated in DHTO versus DH all involved protein synthesis or trafficking and had reciprocal upregulation in DH versus control. The dashed line represents statistical significance. (D) The top ten upregulated processes in DHTO versus DH also had reciprocal downregulation in DH versus control. Similar to the list of top regulated processes using differentially regulated genes in all groups, epidermal growth factor receptor and ephrin signaling (axon development) were represented, but two of the top three processes involved small GTPases (cell leading edge and lamellipodium).