Skip to main content
. 2016 Mar 18;23(9):1458–1470. doi: 10.1038/cdd.2016.27

Figure 5.

Figure 5

Methylation activities of Mili and Dnmt3a. (a) MSP analysis indicated that injection of Mili or Dnmt3a into 100 zygotes enhanced methylation levels of retrotransposon LINE1, MuERV, and IAP. *P<0.05. **P<0.01. Error bars, S.D. (n=4). (b) miR-17 zygotes were microinjected with 2 pl control 1 (PBS), control 2 (mouse Ig), Mili, Dnmt3a, and Mili+Dnmt3a, incubated for 8 h, followed by real-time PCR. Injection with Mili or Dnmt3a repressed mRNA levels of LINE1, MuERV, and IAP. *P<0.05. **P<0.01. Error bars, S.D. (n=4). (c) MSP analysis indicated that injection with Mili or Dnmt3a enhanced methylation levels of the miRNA genes in zygotes. *P<0.05. **P<0.01. Error bars, S.D. (n=4). (d) Injection with anti-Mili or anti-Dnmt3a antibody decreased the methylation levels of the miRNA genes in zygotes. *P<0.05. **P<0.01. Error bars, S.D. (n=4). (e) Injection with miR-17-5p mimic repressed methylation levels of miRNA genes in zygotes. *P<0.05. **P<0.01. Error bars, S.D. (n=4). (f) Injection with miR-17-5p-IN enhanced methylation levels of miRNA genes. *P<0.05. **P<0.01. Error bars, S.D. (n=4). (g) Hundred zygotes were collected, lysed, and precipitated with antibody against AGO2. Precipitated AGO2 pulled down more miRNAs in the zygotes of miR-17 transgenic mice. **P<0.01. Error bars, S.D. (n=4)