Figure 5.

GFR signals restrain BMF expression through PI3K/AKT-dependent signals. (a and b) Anoikis resistance in E-cadherin-negative breast cancer cells is AKT dependent. Shown is the quantification of anoikis induction in mILC1, MCF7-ΔCDH1.1 and MCF7-ΔCDH1.2 grown in Sus measured by flow cytometry. Data represent the mean±S.D., n=3, t-test *P<0.05, **P<0.005. (c–e) AKT inhibition results in BMF expression. Shown are quantitative PCR analyses of Bmf/BMF mRNA and western blotting analyses of Bmf/BMF protein levels in mILC1, MCF7-ΔCDH1.1 and MCF7-ΔCDH1.2 cultured in Sus and after treatment with AKTi. Data represent the mean±S.D., n=3, t-test *P<0.05, **P<0.005. Blots are cropped corresponding to the black box