Figure 5.

LMO4 mediates N-RGMa inhibition on growing axons. (a) In situ hybridization with a LMO4 anti-sense (LMO4-AS) probe demonstrated LMO4 is expressed by RGCs in the chick E8 retina. Negative control, sense (LMO4-S). (b) Temporal retinal cells were transfected with an RFP reporter together with an miRNA. RGC axons cultured on N-RGMa appeared longer in the presence of LMO4 miRNAs. (c) Quantification showed that LMO4-miRNAs significantly restored outgrowth on N-RGMa. Outgrowth on Laminin or C-RGMa was not affected by LMO4-miRNAs (P<0.005). (d) RGCs were transfected with a GFP reporter together with control miRNA-RFP, or NeICD-GFP together with miRNAs-RFP. NeICD-GFP transfected axons appeared shorter than GFP. Axonal length in NeICD axons was increased by the presence of LMO4-miRNAs. (e) Quantifications showed that LMO4-miRNAs significantly restored axonal growth in NeICD transfected cells. *P<0.05