Figure 7.

Deletion of Rint1 in progenitors of dorsal telencephalon cause severe defects already at E15.5. (a) TUNEL and Activated Caspase 3 staining of Rint1^Ctrl and Rint1^Emx1Cre cortex at 15.5 exhibits extensive apoptosis not only in CP but also in VZ/SVZ as shown in the quantification in the right panel. (b) Destabilization of Golgi (GM130) and ER (PDI) homeostasis in Rint1^Emx1Cre cortices with quantification of Golgi fragmentation and ER vacuolization in the bottom panel. Levels of ER stress marker CHOP (c) as well as autophagosome marker LC3B-II (d) are increased in Rint1^Emx1Cre cortex. (e) Quantification of LC3B-II/LC3B-I and LC3B-II/Actin ratios in cortex from three different embryos. (f) p62 and LAMP2 accumulation in apoptotic active Caspase3-negative cells in Rint1^Emx1Cre cortex at E15.5 with statistic evaluation in the right panel. Stars indicate significance in two tailed Student's t-test **P<0.005, ***P<0.0005