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. 2015 Sep 18;23(3):469–483. doi: 10.1038/cdd.2015.114

Figure 1.

Figure 1

TNFα induces PML protein accumulation without changing protein stability. Accumulation of PML protein (a) and mRNA (b) during TNFα stimulation in HUVECs. HUVECs were treated with TNFα (20 ng/ml) and harvested at the indicated times. The whole-cell lysates and total RNA were prepared for western blotting with the indicated antibodies and qRT-PCR, respectively. β-Actin protein and 18S rRNA were used as an internal control for western blotting and qRT-PCR, respectively. (c and d) The effect of TNFα on PML protein expression in HeLa cells. The experiments were similar to those in (a) and (b) except HeLa cells were used. (d) Quantitative measurement of the TNFα effect on PML protein and mRNA accumulation (n=3). (e) The effects of TNFα on PML protein half-life in HeLa cells were measured as described in the Materials and Methods. (f) The half-life of PML protein with or without TNFα treatment (20 h) was derived from three experiments. All the results shown in graphs are mean±S.D.