Figure 2.

Steroid hormones binding factors expression is p53-dependent and augmented upon DNA damage. (a) HepG2 sh-con/sh-p53 cells were treated with 10 μM Nutlin-3a for 6, 16, 24 and 48 h. The mRNA levels of SHBG, CYP21A2 and CBG were measured by qRT-PCR. (b) Western blot analysis of SHBG and p53 protein levels in the same cells as in a. GAPDH was used as loading CON. (c) HepG2 sh-con/sh-p53 cells were treated with 0.5 μg/ml DOX for 16 and 24 h. mRNA levels of SHBG, CYP21A2 and CBG were measured by qRT-PCR analysis. (d) Western blot analysis of SHBG and p53 protein levels in the same cells as in c. β-Actin was used as loading CON. Results are of a representative experiment from at least three experiments. *P<0.05, **P<0.01, determined by Student's t-test analysis