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. 2016 Oct 14;9:357–366. doi: 10.2147/CCID.S111999

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© 2016 Jensen et al. This work is published and licensed by Dove Medical Press Limited

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mRNA levels of the ARE genes Nqo1 (A), Gclm (B), Gclc (C), and Hmox1 (D) were quantified using qPCR analysis.

Notes: For this testing, HaCaT cells were plated at 350,000 cells/well in six-well plate formats. Compounds were added 18–20 hours after plating; cells were harvested for qPCR analysis 6 hours after treatment. Data are expressed as fold change relative to the DMSO-only control condition. WSEM significantly increased mRNA levels of all the four ARE antioxidant response genes evaluated (P<0.05). All treatments induced statistically significant differences compared to the DMSO-only control (*P<0.05). Data are representative of two independent experiments.

Abbreviations: ARE, antioxidant response element; Bardox, bardoxolone; DMF, dimethylfumarate; DMSO, dimethyl sulfoxide; mRNA, messenger RNA; qPCR, quantitative polymerase chain reaction; WSEM, water-soluble egg membrane.