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. Author manuscript; available in PMC: 2017 Oct 6.
Published in final edited form as: Cell. 2016 Sep 29;167(2):419–432.e16. doi: 10.1016/j.cell.2016.09.011

Figure 4. Customized Killer T Cells – synNotch Driven TRAIL Production.

Figure 4

(A) CD8+ cytotoxic T cells recognize infected cells via their TCR and directly kill the infected cell by creating pores in the cell with perforin allowing for the delivery of granzymes that initiate programmed cell death.

(B) CD4+ T cells were engineered with the α-GFP synNotch controlling the expression of the apoptotic regulator TRAIL in response to surface GFP.

(C) Histograms showing the selective expression of surface TRAIL after 24 hours of CD4+ α-GFP synNotch T cell incubation with surface GFP+ K562s.

(D) Histograms showing surface GFP+ K562 cell death via uptake of the dead stain SYTOX blue after 24hr. co-culture with the indicated T cell type (T cell:Target Cell Ratio = 1:1).

(E) Percentage target cell survival calculated from replicate data shown in panel D (n=4, error bars = SEM, significance determined by Student’s t-test ** p≤0.01)