Figure 1.

Cas9-mediated indel formation and DNA sequencing. (A) T7E1 mismatch detection assays demonstrating frequency of indel formation (%indel) for each gRNA targeting VEGF-A, expressed in mean ± standard deviation. Lentiviral vectors expressing SpCas9 without VEGF-targeted gRNAs were used as controls. Representative results are shown from four total independent experiments. (B) Sanger sequencing chromatograms showing indel formation at the predicted cut site for each target site. (C) Representative deep sequencing results for V-1 confirming indel formation at the predicted cut site (red arrowhead), including insertions (bases in red) and deletions (dashes).