Table 1. Primers and probes used in multiplex real-time PCR to detect RSV-A, RSV-B, and human ACTB.

Target a	Primers and probes	Sequences b
RSV-A
	Forward	5’-ACTGCAATCAYACAAGATGCAACRA-3’
	Reverse	5’-CAGATTGRAGAAGCTGATTCCA-3’
	Probe	5’-FAM-CCAGATCAAGAACACAACCCCARCATACCT-BHQ1-3’
RSV-B
	Forward	5’-ACTTACCTTACTCAAGTCTCACCAGAAA-3’
	Reverse	5’-TTGTRGCTGARTTTGTGTGGAT-3’
	Probe	5’-Texas red-TTAGCCCATCCMAACAAYCCACAACC-BHQ2-3’
Human beta actin
	Forward	5’-TGGCACCCAGCACAATGA-3’
	Reverse	5’-GCCGATCCACACGGAGTACT-3’
	Probe	5’-JOE-ATCAAGATCATTGCTCCTCCTGAGCGC-BHQ1-3’

Note:

^a Conserved G gene specific for RSV-A and RSV-B, and human ACTB gene were used for detection.

^b TaqMan probes were used; Y represents C or T; R represents A or G; M represents A or C.