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. 2016 Oct 20;11(10):e0165030. doi: 10.1371/journal.pone.0165030

Fig 7. RAP2.4a localization in leaf cells and its effect on stomatal aperture.

Fig 7

A) Immunocolocalization studies show the localization of GFP-35S::CpRAP2.4a in transgenic plants in nucleus and sieve elements. Lignin from sieve elements shows autofluorescence at 480nm but not at 620 nm. B) SEM of stomatal aperture in wild type tobacco plants versus transformed tobacco plants (35S::CpRap2.4a). Arrows show stomata in blue (open) and red (close) for wild type and GFP-35S::CpRAP2.4a in transgenic plants in presence or absence of ABA. Yellow arrows indicate rounded closed stomata only seen in wild type plants in the presence of 50 μM ABA. C) Phenotype of Stomata of leaves from the abaxial epidermis of the wild type tobacco plants (Wt) versus transformed tobacco plants (35S::CpRap2.4a) in presence or absence of ABA.