Table 2. List of detection primers designed in this study.
| Cowpea viruses | Primers | Sequences | Gene | Annealing temperature (°C) | Extension duration (Sec) | Amplicon length (pb) |
|---|---|---|---|---|---|---|
| Cowpea polerovirus 1 and | PoleroNB3897F | GAGTTCATCTCCGAGGCC | cp | 55 | 30 | 263 |
| Cowpea polerovirus 2 | PoleroNB4160R | CDTCTACCTATTTSGGRTTHTG | ||||
| SCPMV | SCPMVNB2698F | CTGGGARTTRTGGGCTGATG | RdRp | 63 | 60 | 721 |
| SCPMVNB3419R | CTGAGCAATAGGGGCCATG | |||||
| SCPMVNB2783F | TCRTGYTTCATGAACTCAGTC | 53 | 30 | 133 | ||
| SCPMVNB2916R | AGYTCAGCCATRAGGCAWCG | |||||
| CPMoV | CPMoV1138F | TGAGYACTTTCATCAAAGCWGA | RdRp | 53 | 60 | 548 |
| CPMoV1686R | ACACARTCRTCWCCGTTGTT | |||||
| CPMoV1138F | TGAGYACTTTCATCAAAGCWGA | 51 | 30 | 455 | ||
| CPMoV1593R | GTGTTCATRTCMCCACTCAT | |||||
| Cowpea tombusvirid 1 | Tombus3NB31F | CAAGGTTCGACCAACATGTG | RdRp | 57 | 30 | 412 |
| Tombus4NB79R | CCAGTTTACAACCTTGAGGAG | |||||
| Tombus2NB237F | TGTCTCTCGTGCCGATGCT | 55 | 30 | 308 | ||
| Tombus3NB52R | GGTTCGACCAACATGTGGG | |||||
| Tombus2NB237F | TGTCTCTCGTGCCGATGCT | RdRp/cp | 55 | 120 | 1772 | |
| Tombus1NB44R | CCTGGTGTCGATGTGGCC | |||||
| Tombus3NB31F | CAAGGTTCGACCAACATGTG | 55 | 90 | 1485 | ||
| Tombus1NB44R | CCTGGTGTCGATGTGGCC | |||||
| Cowpea tombusvirid 2 | Tomb2NB50F | CTGTGTGCTGTTCGTGGAG | RdRp | 55 | 30 | 122 |
| Tomb2NB172R | TCAATCTTCTCTATATCGTAAAC | |||||
| Cowpea tombusvirid 3 | Tomb1NB18F | TATCGGGGAGCGTTTGTACA | RdRp | 55 | 30 | 175 |
| Tomb1NB193R | TGCATGTCGGGTGTAATACC | |||||
| Cowpea associated mycotymovirid 1 | TymoNB120F | CTTTGGGTAGCACTATCCAC | RP | 55 | 30 | 295 |
| TymoNB415R | GAGTTTTGCTCCTTGAGACG | |||||
| TymoNB42F | GCTGCCATAGAAAAGCGCC | RP | 55 | 30 | 154 | |
| TymoNB196R | TAAAGAAGCTCGTCGAAGGG |
cp: coat protein; RdRp: RNA dependant RNA polymerase; RP: replication-associated polyprotein