Fig 1. Short-chain polyphosphates accelerate the inhibition of TFPIα by FXIa.

rTFPI (10 nM) was incubated with 2 nM FXIa for 10, 15, or 30 min in the presence or absence of 25 μM Zn²⁺,5 mM Ca²⁺, 10 μM SCP, Ca²⁺ and SCP or Zn²⁺ and SCP. rTFPI was analyzed by western blotting, with a polyclonal anti-TFPI antibody under non-reducing conditions. The bands were quantified by densitometry. Data are mean ± SE (n = 3).