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(A) 96-well plates were coated with 5 μg/ml TFPIα (○,●) or BSA (◻) and increasing concentrations of biotinylated-polyphosphate (bio-polyP) (◻, ○,●) was added to selected wells. Selected experiments were done in the presence of 100 μM SCP (●). Binding was detected with streptavidin-HRP. Data are mean ± SE (n = 3).
