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. 2016 Oct 20;11(10):e0165083. doi: 10.1371/journal.pone.0165083

Fig 1. HSA interacts with GML via high affinity binding to the saturated 12 carbon chain.

Fig 1

(A) 5μM of HSA were purified via gel filtration and placed in the sample chamber of the ITC. 150 μM of ethanol solubilized GML was placed in the syringe and injected into the sample chamber twenty one times with 180 seconds interval between injections. Top graph shows the change of power with each GML injection and bottom graph shows data fitted with a single binding site model for GML and HSA. (B) 5μM of gel filtrated HSA was placed in the sample chamber of the ITC and 150 μM of ethanol solubilized lauric acid (LA) was placed in the syringe that was injected into the sample chamber twenty one times with 180 seconds interval between injections. Top graph shows the change of power with each LA injection and bottom graph shows data fitted with a single binding site model for GML and LA. (C) 5μM of gel filtrated HSA was placed in the sample chamber of the ITC and 150 μM of glycerol was placed in the syringe that was injected into the sample chamber twenty one times with 180 seconds interval between injections. Top graph shows the change of power with each glycerol injection and bottom graph shows data fitted with a single binding site model for GML and glycerol.