Table 1. Thermodynamics, stoichiometry, and dissociation constant of albumin with GML, lauric acid or glycerol.

	ΔH (kcal/mol)	n	Kd (μM)
GML + HSA	-3.0 ± 1.1	2.6 ± 0.9	1.4 ± 0.95
GML + BSA	-4.5 ± 0.77	1.7 ± 0.7	2.2 ± 1.1
GML + PSA	-2.0 ± 0.63	3.1 ± 0.2	4.5 ± 1.6
GML + RSA	-4.5 ± 0.61	2.8 ± 0.6	5.4 ± 1.3
GML + MSA	-4.9 ± 1.1	2.6 ± 0.3	6.3 ± 2.7
LA + HSA	-24 ± 0.63	1.1 ± 0.4	4.9 ± 1.1
Glycerol + HSA	N.D.	N.D.	>50

Table 1: Thermodynamics, stoichiometry, and dissociation constant of the interaction of GML, lauric acid (LA) or glycerol with human serum albumin (HSA), bovine serum albumin (BSA), pig serum albumin (PSA), rabbit serum albumin (RSA), and mouse serum albumin (MSA). Data generated from ITC experiments were fitted into a single site binding model provided within the ITC software analysis package. Three independent experiments were averaged to produce above values for enthalpy (ΔH), stoichiometry (n), and dissociation constant (Kd). N.D. means not detectable.