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. 2016 Oct 20;11(10):e0164543. doi: 10.1371/journal.pone.0164543

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© 2016 dos Santos et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — Immunofluorescence assays were performed to determine the number of T cell markers (CD4, CD8 and CD45RA) expressing or not CLA. Representative images presented CD45RA+ (red, Alexa Fluor 633; upper right panels) and CLA+ (green, Alexa Fluor 488; lower right panels), colocalization (yellow, white arrow head and insert; left panels). The nuclei (blue) were stained using DAPI. Scale bar = 50 mm. Data are representative of 3 specimens of each group, being one T1R (A) and one BL patient (B). Images were visualized and obtained by a Zeiss Colibri fluorescent microscope. The graph shows single and double-positive CD4+/CLA+, CD8+/CLA+ and CD45RA+/CLA+ cells in T1R and BL skin infiltrates (C). *p<0.05 and **p<0.01 (Mann-Whitney test; group comparisons).