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(A) Gene disruption strategy for MoAbfB (MGG_06843T0). HYG, hygromycin B phosphotransferase marker gene cassette. (B) Genomic DNA samples from a wild-type strain and 3 ΔMoAbfB mutants were digested with BamHI and probed with the 5’-flanking 1,284 bp of the MoAbfB gene. (C) RT-PCR to confirm the loss and recovery of the MoAbfB transcript.
