Fig 3. Pressure effects on pH and Ca²⁺-binding to EGTA.

Pressure-dependence of pH due to pressure effects on HEPES according to Eq (14) [17] using the Δ_iv determined by Kitamura et al. [16]. The development of the equilibrium constant of EGTA binding to Ca²⁺ is estimated by Eq (16) using the Δ_rv determined by Hasselbach et al. [15].