Table 3. Differentially expressed genes in laboratory-evolved strains compared with wild-type and clinical strains.
| Gene | Gene | Product | XH210 | XH211 | XH212 | XH213 | XH214 | XH215 | XH216 |
|---|---|---|---|---|---|---|---|---|---|
| up-regulated | |||||||||
| LQ47_01505 | phospholipid ABC transporter substrate-binding protein | 2.2* | 4.0 | 4.3 | 3.5 | 3.4 | 3.5 | 3.8 | |
| LQ47_01510 | ABC transporter substrate-binding protein | 2.1* | 4.0 | 4.2 | 3.9 | 3.1 | 3.6 | 4.1 | |
| LQ47_01515 | phospholipid ABC transporter substrate-binding protein | 2.8* | 4.5 | 4.7 | 4.5 | 4.1 | 4.5 | 4.9 | |
| LQ47_08715 | nitrate/nitrite sensor protein NarX | -0.5 | 3.0 | 2.9 | 3.5 | 3.0 | 2.7 | 2.9 | |
| LQ47_08720 | nitrate/nitrite transporter NarK | -1.1 | 6.2 | 4.9 | 5.5 | 4.9 | 5.2 | 5.9 | |
| LQ47_08730 | nitrate reductase | -0.7 | 5.1 | 4.5 | 5.2 | 4.6 | 4.3 | 5.1 | |
| LQ47_08735 | nitrate reductase | -0.3 | 5.3 | 4.5 | 5.5 | 4.8 | 4.3 | 5.3 | |
| LQ47_08740 | nitrate reductase | -1.0 | 4.4 | 4.3 | 4.9 | 4.9 | 3.9 | 4.8 | |
| LQ47_08745 | nitrate reductase | 2.6* | 7.1 | 6.6 | 7.1 | 6.7 | 6.5 | 7.3 | |
| LQ47_16215 | sensor protein BasS/PmrB | 1.9* | 4.0 | 3.3 | 4.2 | 3.9 | 3.8 | 4.0 | |
| LQ47_22580 | transcriptional regulator | 3.6* | 4.9 | 6.4 | 5.0 | 4.9 | 5.1 | 5.6 | |
| down-regulated | |||||||||
| LQ47_02160 | hypothetical protein | -1.1* | -10.4 | -6.8 | -5.9 | -6.7 | -7.4 | -8.9 | |
| LQ47_04290 | hydrogenase 3 membrane subunit | -0.5 | -4.9 | -4.1 | -6.3 | -6.8 | -5.9 | -4.7 | |
| LQ47_04295 | hydrogenase 3 large subunit | -0.2 | -5.2 | -4.5 | -6.2 | -6.7 | -5.9 | -4.9 | |
| LQ47_04310 | formate hydrogenlyase maturation protein HycH | -0.6 | -5.5 | -3.7 | -6.2 | -5.9 | -6.1 | -4.6 | |
| LQ47_04315 | hydrogenase 3 maturation protease | -0.2 | -6.8 | -5.5 | -5.6 | -10.9 | -10.4 | -5.6 | |
| LQ47_04680 | fimbrial protein | 0.1 | -5.5 | -8.2 | -7.1 | -5.7 | -10.8 | -7.1 | |
| LQ47_09405 | formate dehydrogenase | -0.3 | -5.8 | -4.0 | -5.2 | -5.4 | -4.7 | -5.0 | |
| LQ47_09545 | acetoin reductase | -0.5 | -7.5 | -4.8 | -7.5 | -6.4 | -7.8 | -7.1 | |
| LQ47_09550 | acetolactate synthase | -0.4 | -6.3 | -4.5 | -6.3 | -7.9 | -6.7 | -5.3 | |
| LQ47_11630 | hypothetical protein | -3.9* | -5.1 | -10.3 | -7.7 | -10.3 | -6.2 | -7.8 | |
| LQ47_12010 | methionine synthase | -2.4 | -5.4 | -8.5 | -5.1 | -7.4 | -6.3 | -6.0 | |
| LQ47_23900 | 5,10-methylenetetrahydrofolate reductase | -1.5 | -4.8 | -5.7 | -6.0 | -4.6 | -6.1 | -5.2 | |
| Common | |||||||||
| LQ47_04775 | acrA | acriflavin resistance protein AcrA | 14.9 | 19.3 | 14.3 | 15.1 | 17.5 | 15.3 | 16.8 |
| LQ47_10655 | gsiA | glutathione ABC transporter ATP-binding protein | 16.4 | 6.3 | 6.2 | 7.0 | 6.9 | 13.4 | 14.6 |
| LQ47_17285 | entE | enterobactin synthase subunit E | 10.6 | 16.5 | 17.2 | 10.2 | 10.8 | 19.9 | 18.2 |
| LQ47_17585 | ramA | transcriptional regulator | 5.2 | 6.3 | 5.3 | 7.1 | 5.7 | 6.8 | 8.2 |
| LQ47_17590 | romA | beta-lactamase | 6.4 | 14.3 | 15.2 | 13.6 | 14.1 | 6.8 | 8.0 |
| LQ47_17595 | ramR | TetR family transcriptional regulator | 4.9 | 3.6 | 5.1 | 4.9 | 6.0 | 4.3 | 4.9 |
| LQ47_22005 | membrane protein | 4.2 | 3.8 | 5.1 | 5.3 | 3.6 | 5.3 | 4.9 |
*: differentially expressed genes in XH210, clinical isolate.