Table 4. Complementation experiment.
TGC MIC (mg/L) | Wild type | pCR2.1-T vector | pCR2.1-ramR | pCR2.1-lon | pCR2.1-lon-ramR | |
---|---|---|---|---|---|---|
Strain | Genotype | |||||
XH210 | ramR Q122* | 8 | 8 | 4 | ||
XH211 | ramA Q72L lon Q317* ramR Δ190 bp (322–511) | 128 | 64 | 16 | 4 | 16 |
XH212 | rpsJ V57L lon D445V PramR +G | 256 | 256 | 256 | 128 | 64 |
XH213 | ramR A40T lon R33W rpoC Δ18 bp (634–651) | 128 | 128 | 4 | 64 | 32 |
XH214 | ramR L58P rpoC G336A | 64 | 64 | 8 | ||
XH215 | ramR Q135* lon Δ9 bp (791–799) rpoC S263Y | 256 | 128 | 8 | 16 | 32 |
XH216 | ramR S29* lon N417K | 64 | 64 | 32 | 64 | 32 |
Note:
*: stop codon;
Δ: deletion; bp: base pair; NA: no mutation.