. 2016 Oct 20;11(10):e0165099. doi: 10.1371/journal.pone.0165099

Table 1. Top 20 proteins most commonly identified by label-free shotgun proteomics in Piscirickettsia salmonis membrane vesicles.

LF-89		NVI 5692		NVI 592
Identified protein	Total number of spectra	Identified protein	Total number of spectra	Identified protein	Total number of spectra
Outer membrane family protein	81	Putative uncharacterized protein^*	144	Putative uncharacterized protein^*	171
DNA-directed RNA polymerase subunit beta	79	Putative uncharacterized protein^**	136	Peptidyl-prolyl cis-trans isomerase^**	140
Bacterial DNA-binding family protein^*	72	Prolyl oligopeptidase family protein^**	133	Outer membrane beta-barrel domain protein^**	104
Chaperone protein DnaK^*	63	Type I secretion outer membrane TolC family protein^**	89	Outer membrane protein assembly factor BamA^**	89
60kDa chaperonin GroEL	58	SH3 domain of the SH3b1 type family protein^*	86	Prolyl oligopeptidase family protein^**	83
30s ribosomal protein S1^*	44	Conjugal transfer/type IV secretion DotA/TraY family protein^**	81	Type I secretion outer membrane, TolC family protein^**	82
SH3 domain of the SH3b1 type family protein^*	44	Outer membrane beta-barrel domain protein^**	81	Outer membrane family protein	79
ATP synthase subunit beta	40	Outer membrane protein assembly factor BamA^**	72	Chaperone protein DnaK^*	74
Succinyl-CoA synthetase subunit beta	37	30s ribosomal protein S1^*	69	SH3 domain of the SH3b1 type family protein^*	72
Adenylosuccinate synthetase	35	Peptidyl-prolyl cis-trans isomerase^**	65	Conjugal transfer family protein^**	71
50S ribosomal protein L2^*	35	Bacterial DNA-binding family protein^*	65	OmpA family protein	68
Pyruvate dehydrogenase E1 component	35	50S ribosomal protein L2^*	64	Outer membrane protein assembly factor BamD^**	64
Translation elongation factor Tu	32	Outer membrane protein assembly factor BamD^**	63	Bacterial DNA-binding family protein^*	62
ATP synthase subunit alpha	31	Chaperone protein HtpG	60	Conjugal transfer/type IV secretion DotA/TraY family protein^**	61
30S ribosomal protein S10	31	Conjugal transfer family protein^**	60	Glycerophosphoryl diester phosphodiesterase family protein^**	57
Acetyl-CoA carboxylase, biotin carboxylase subunit	30	NAD-specific glutamate dehydrogenase	59	50S ribosomal protein L2^*	57
GTP-binding protein TypA/BipA	30	ATP synthase subunit alpha	57	30s ribosomal protein S1^*	55
Adenylosuccinate lyase	30	Chaperone protein DnaK ^*	57	Putative uncharacterized protein	52
Putative uncharacterized protein^*	29	Glycerophosphoryl diester phosphodiesterase family protein^**	56	ostA-like family protein	48
Glutamine synthetase	28	Succinyl-CoA synthetase subunit beta	50	DSBA-like thioredoxin domain protein	46

*Proteins identified in MVs from all three strains of P. salmonis

**Proteins identified in MVs from P. salmonis strains NVI 5692 and NVI 5892