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. 2016 Oct 20;11(10):e0164789. doi: 10.1371/journal.pone.0164789

Fig 4. Monitoring DS-Cav1 stability with 4D7.

Fig 4

(A) DS-Cav1 was stored at -70°C or at 4°C for either 14 or 102 days, and surface plasmon resonance was used to assess protein binding to 4D7 (left panel) and D25 (right panel). DS-Cav1 stored at -70°C and thawed immediately before use (cyan line) or stored at 4°C for 14 (blue line) or 102 (green line) days was flowed over the surface of 4D7- or D25-coated sensor chip channels, and response units over time, in seconds, were plotted. (B) Sandwich ELISA. DS-Cav1 stored for approximately 5 months at 4°C was captured on an ELISA plate coated with 4D7. The captured 4D7-reactive protein was bound by palivizumab (orange line), which recognizes both prefusion and postfusion forms of RSV F. In contrast, the captured 4D7-reactive protein was not bound by any of the prefusion-specific monoclonal antibodies tested, such as D25 (site Ø), MPE8 (site III), or AM14 (site V).