Table 2. Intra-day and inter-day precision and accuracy of the proposed methods.
In Human Plasma matrix | LQC (20 ngmL-1) | MQC (150 ngmL-1) | HQC (300 ngmL-1) | |||
Intra-day assay* | Inter-day assay** | Intra-day assay | Inter-day assay | Intra-day assay | Inter-day assay | |
Mean | 19.70 | 19.89 | 147.95 | 147.72 | 299.21 | 299.48 |
Standard deviation (SD) | 0.31 | 0.50 | 1.67 | 1.61 | 3.16 | 3.66 |
Precision (%RSD) | 1.58 | 2.54 | 1.13 | 1.09 | 1.06 | 1.22 |
Accuracy (%RE) | -1.48 | -0.55 | -1.37 | -1.52 | -0.26 | -0.17 |
In RLMs matrix | LQC (20 ngmL-1) | MQC (150 ngmL-1) | HQC (300 ngmL-1) | |||
Intra-day assay* | Inter-day assay** | Intra-day assay | Inter-day assay | Intra-day assay | Inter-day assay | |
Mean | 19.67 | 19.97 | 147.75 | 147.88 | 299.1 | 299.07 |
Standard deviation (SD) | 0.29 | 0.46 | 1.59 | 1.86 | 3.08 | 2.90 |
Precision (%RSD) | 1.49 | 2.31 | 1.07 | 1.26 | 1.03 | 0.97 |
Accuracy (%RE) | -1.65 | -0.16 | -1.05 | -1.42 | -0.3 | -0.31 |
* Average of twelve determinations of day 1.
** Average of six determinations in three consecutive days.