Figure 3.

MluCI digest of the 150-bp PCR amplimer from exon 12 that contains the C→T mutation (Arg577→ stop) in a normal dog (N), the subject of this study (S), and a heterozygous carrier for the Arg577→stop mutation (Het). The mutation creates an MluCI cleavage site, resulting in digest fragments of 103 and 47 bp in the subject's DNA, whereas the normal DNA remains undigested, and the DNA from a heterozygous female dog yields fragments corresponding to both undigested (normal) and digested (hemophilia A) DNA sequences.