Table 2.

Oligonucleotide primers used to amplify genomic DNA and create cDNA from hemophilia A and normal dogs

Primer location	Primer sequence	Amplimer size (bp)
Canine factor VIII exon 22	5′-GATCTCTTGGCACCGATGAT-3′	236
Canine factor VIII exon 23	5′-TGCAAACGGATGTACTGAGC-3′
Canine factor VIII exon 22	5′-TCCAGCCTCTACGTGTCTCA-3′	200
ch8 (sequence following exon 22 in intron 22 inversion)	5′-TGGCTTTAAGCAGTGGACCT-3′
Canine factor VIII exon 12	5′-TGATGTCAGACAAGAGAAATGTCA-3′	150
Canine factor VIII exon 12	5′-TGTGCATGATGTTAGAGAGTTGG-3′